Centromeric index measurement by slit-scan flow cytometry

Signal processing in slit-scan flow cytometry of cell conjugates.

The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells. Preliminary measurements of changes in the membrane potential induced by interactions between natural killer (NK) cells and their target cells are presented.

Measurement of Soluble Biomarkers by Flow Cytometry

Microparticle based flow cytometric assays for determination of the level of soluble biomarkers are widely used in several research applications and in some diagnostic setups. The major advantages of these multiplex systems are that they can measure a large number of analytes (up to 500) at the same time reducing assay time, costs and sample volume. Most of these assays are based on antigen-ant...

EMOSS: an epiillumination microscope objective slit-scan flow system.

An epiillumination microscope objective slit-scan flow system has been fabricated utilizing two dimensional slit scanning with hydrodynamic sample stream focussing. Low resolution (4 micron) analysis of cellular fluorescence is facilitated by the definition of a stabilized flow plane through hydrodynamic focussing. Coincidence of the region of stabilized flow with the focal plane of the microsc...

Measurement of The Nonlinear Refractive Index by Z-scan Technique

In this seminar the third-order nonlinear optical phenomena are described. The emphasis is on the nonlinear refractive index n2. We describe the Z-scan technique, which is a simple method to determine n2. At the end we show how Z-scan method can be used to examine protein concentration in human blood.

Improved measurement of calcium mobilization by flow cytometry.